Anti-Amyloid-beta A4 protein APP Antibody

Product Name

Anti-Amyloid-beta A4 protein APP Antibody

View all APP Antibodies

SKU/Catalog Number

A00081

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Amyloid-beta A4 protein APP Antibody (Catalog # A00081). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

APP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Amyloid-beta A4 protein APP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00081)

Host

Rabbit

Contents

APP Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

APP antibody was raised against a peptide corresponding to 10 amino acids of human amyloid A4 protein precursor (APP) corresponding to the amino terminus of the 4K Ab peptide generated by beta- and gamma-secretases. The immunogen is located within amino acids 650 - 700 of APP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.

Reactive Species

A00081 is reactive to APP in Human, Mouse, Rat

Applications

A00081 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

86.943kDa

Background of APP

Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

APP antibody can be used for detection of APP and Ab peptide by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of APP in (A) human

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Immunohistochemistry of APP in human brain (Alzheimer’s disease) tissue with APP antibody at 10 μg/mL.

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Immunohistochemistry of APP in human brain tissue with APP antibody at 2.5 μg/ml.

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Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 μg/mL.

Green: APP Antibody (A00081)
Blue: DAPI staining

Gene/Protein Information For APP (Source: Uniprot.org, NCBI)

Alternative Names

Abeta; Amyloid beta; beta-Amyloid Peptide APP AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, APP amyloid beta precursor protein amyloid-beta precursor protein|alzheimer disease amyloid protein|amyloid beta (A4) precursor protein|amyloid beta A4 protein|amyloid precursor protein|beta-amyloid peptide|beta-amyloid peptide(1-40)|beta-amyloid peptide(1-42)|beta-amyloid precursor protein|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II|testicular tissue protein Li 2

For more info on APP, check out the APP Infographic

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In this infographic, you will see the following information for APP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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